Composite

Part:BBa_K2142000:Design

Designed by: Lukas Rieder   Group: iGEM16_NAWI-Graz   (2016-09-15)


Composite part containing mazE and red chromoprotein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 815
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We were working with different antitoxin/toxin systems. To be sure the E.coli will survive its own toxin we had to express the antitoxin constitutive and also the chromoprotein had to be expressed without inducing. The bidirectional double terminator in the end allows us to use only one terminator to safe some space on the plasmid.


Source

This part was made with sequences of the registry and from NCBI database.

References